Oktober 2017 - present

PhD Medical Life Science & Technology

Technische Universität München

September 2014 - Mai 2017

Life Science Research Professional, Stanford University

September 2013 - August 2014

MSc Burns, Plastic and Reconstructive Surgery, University College London

Thesis title: Characterization of 'layer-by-layer' fibroblast constructs as tissue engineered dermal substitutes

Juni 2013 - September 2013

Contractor, Vivasure Medical Ltd, Galway, Ireland

Mai 2012 - September 2012

Researcher, Department of Anatomy, NUI Galway, Ireland

Juni 2011 - August 2011
Stagecraft Counselor, Camp Taconic, Hinsdale Massachusetts

September 2009 - Juni 2013

BSc Biomedical Science, National University of Ireland, Galway

Thesis title: The effect of Rac1 knockout in a model of contact irritant dermatitis

RNA extraction from cells/tissue

RNADNA quantification and purification

RT, qPCR and gene analyses

In vivo soft tissue fat grafting, renal capsule injections, calvarial defect/bone harvest

small animal dosing

in vivo LDA, CT and bioluminescent imaging

Brett E, Zielins ER, Luan A, Ooi CC, Shailendra S, Atashroo D, Menon S, Blackshear C, Flacco J, Quarto N, Wang SX, Longaker MT, Wan DC. Magnetic Nanoparticle-Based Upregulation of Bcl-2 Enhances Bone Regeneration, Stem Cells Translational Medicine, July 2016, PMID: 27484867

Brett E, Zielins ER, Chin M, Januszyk M, Blackshear CP, Findlay M, Momeni A, Gurtner GC, Longaker MT, Wan DC. Isolation Of CD248-Expressing Stromal Vascular Fraction For Targeted Improvement Of Wound Healing, Wound Repair and Regeneration, April 2017, PMID: 28464475

Brett E, Flacco J, Blackshear C, Longaker MT, Wan DC. Biomimetics of Bone Implants: the Regenerative Road, Bioresearch Open Access, PMCID: PMC5248549, January 2017

Brett E, Tevlin R, McArdle A, Seo EY, Chan CKF, Wan DC, Longaker MT. Human Adipose-derived Stromal Cell Isolation Methods And Use In Osteogenic And Adipogenic In Vivo Applications, Current Protocols, Feb 2017, PMID: 29140567

Brett E, Chung N, Leavitt WT, Momeni A, Longaker MT, Wan DC. A Review of Cell-Based Strategies for Soft Tissue Reconstruction, Tissue Engineering Part A, March 2017, PMID: 28372485

Luan A, Hu MS, Leavitt T, Brett EA, Wang KC, Longaker MT, Wan DC. Non-Coding RNAs in Wound Healing: A New and Vast Frontier, Advanced Wound Care, Jan 2018, PMID: 29344431

Blackshear CP, Rector MA, Chung NN, Irizarry DM, Flacco JS, Brett EA, Momeni A, Lee GK, Longaker MT, Wan DC. Three Dimensional Ultrasound Versus Computerized Tomography in Fat Graft Volumetric Analysis, Annals of Plastic Surgery, July 2017, PMID: 28678028

Luan A, Zielins ER, Wearda T, Atashroo DA, Blackshear CP, Raphel J, Brett EA, Flacco J, Alyono MC, Momeni A, Heilshorn S, Longaker MT, Wan DC. Dynamic Rheology for the Prediction of Surgical Outcomes in Autologous Fat Grafting, Plastic and Reconstructive Surgery, May 2017, PMID: 28574947

Blackshear CP, Flacco JS, Vistnes SM, Chung NN, Irizarry D, Brett EA, Yen DJ, Momeni A, Longaker MT, Wan DC. Cell-Based Soft Tissue Reconstruction in a Hydrogel Scaffold, Annals of Plastic Surgery, June 2017, PMID: 28672889

Marshall CD, Zielins ER, Brett EA, Blackshear CP, Hu MS, Leavitt T, Barnes LA, Lorenz HP, Longaker MT, Wan DC. Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model, JoVE, PMID:28287559, Feb 2017

Zielins ER, Paik K, Ransom RC, Brett EA, Blackshear CP, Luan A, Walmsley GG, Atashroo DA, Senarath-Yapa K, Momeni A, Rennert R, Sorkin M, Seo EY, Chan CK, Gurtner GC, Longaker MT, Wan DC. Enrichment of Adipose Derived Stromal Cells for BMPR1A Facilitates Enhanced Adipogenesis, Tissue Engineering Part A, Feb 2016, PMID: 26585335.

Duscher D, Atashroo D, Maan ZN, Luan A, Brett EA, Barrera J, Khong SM, Zielins ER, Whittam AJ, Hu MS, Walmsley GG, Pollhammer MS, Schmidt M, Schilling AF, Machens HG, Huemer GM, Wan DC, Longaker MT, Gurtner GC. Ultrasound Assisted Liposuction Does Not Compromise the Regenerative Potential of Adipose Derived Stem Cells, Stem Cells Translational Medicine, Jan 2016, PMID: 26702129

Duscher D, Luan A, Rennert RC, Atashroo D, Maan ZN, Brett EA, Whittam AJ, Ho N, Lin M, Hu MS, Walmsley GG, Wenny R, Schmidt M, Schilling AF, Machens HG, Huemer GM, Wan DC, Longaker MT, Gurtner GC. Suction assisted liposuction does not impair the regenerative potential of adipose derived stem cells, Journal of Translational Medicine, May 2016, PMID: 27153799

Luan A, Duscher D, Whittam AJ, Paik KJ, Zielins ER, Brett EA, Atashroo DA, Hu MS, Lee GK, Gurtner GC, Longaker MT, Wan DC. Cell-Assisted Lipotransfer Improves Volume Retention in Irradiated Recipient Sites And Rescues Radiation-Induced Skin Changes, Stem Cells, March 2016, PMID: 26661694

Zielins ER, Luan A, Brett EA, Longaker MT, Wan DC. Therapeutic applications of human adipose-derived stromal cells for soft tissue reconstruction; Discov Med. April 2015, PMID:25977187

Zielins ER, Brett EA, Luan A, Hu MS, Walmsley GG, Paik K, Senarath-Yapa K, Atashroo DA, Wearda T, Lorenz HP, Wan DC, Longaker MT. Emerging drugs for the treatment of wound healing; Expert Opin Emerg Drugs. June 2015, PMID:25704608

Tevlin R, McArdle A, Brett E, Chung MT, Paik K, Seo EY, Walmsley GG, Duldulao CR, Atashroo D, Zielins E, Vistnes S, Chan CK, Wan DC, Longaker MT. A Novel Method of Human Adipose-derived Stem Cell Isolation with Resultant Increased Cell Yield, Plastic and Reconstructive Surgery, April 2016, PMID: 27537222

Paik KJ, Maan ZN, Zielins ER, Duscher D, Whittam AJ, Morrison SD, Brett EA, Ransom RC, Hu MS, Wu JC, Gurtner GC, Longaker MT, Wan DC. Short Hairpin RNA Silencing of PHD-2 Improves Neovascularization and Functional Outcomes in Diabetic Wounds and Ischemic Limbs¸ PLOSone, March 2016, PMID: 26967994

Zielins ER, Brett EA, Longaker MT, Wan DC. Autologous Fat Grafting: The Science Behind the Surgery, Aesthetic Surgery Journal, April 2016, PMID: 26961989